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KMID : 0894520090130040217
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Development & Reproduction
2009 Volume.13 No. 4 p.217 ~ p.226
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Development of In Vitro Bioassay for Detection of Estrogenic Activity of Xenobiotics : Monolayer Culture of Hepatocytes using Fish Serum
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Kwon Hyuk-Chu
Maeng Joon-Ho
Kim Eun-Hee
Choi Seong-Hee
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Abstract
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Effects of sera from several fish species on monolayer formation, viability and functions of catfish hepatocytes were investigated to establish a primary hepatocyte culture system for screening endocrine disruptors. Hepatocytes of Korean catfish (Silurus asotus) were attached and formed monolayer using the media supplemented with their own serum or sera from eel and tilapia, but not with fetal bovine serum (FBS). The amount of fish sera (0.5~3%) for monolayer culture of the catfish hepatocytes was less than 1/10 of FBS (5~20%) that is commonly used for primary culture of hepatocytes of other species. The results indicate that FBS can be replaced with sera from some fish species and the fish sera are more effective than FBS in maintaining the shape and functions of the hepatocytes. The primary culture of catfish hepatocytes was maintained monolayer with fish sera for at least 10 days, which makes possible to be used for screening the activities of endocrine disruptors. In conclusion, the primary culture system of hepatocytes with fish sera in the present study could be a useful tool for screening and studying endocrine disruptors.
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KEYWORD
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Catfish(Silurus asotus) hepatocyte, Monolayer culture, Endocrine disruptors, Fish serum, Vitellogenin
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